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KMID : 0613820070170101336
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Journal of Life Science
2007 Volume.17 No. 10 p.1336 ~ p.1340
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Efficient Bulblet Regeneration and Growth from Bulb Scale of Hyacinthus orientalis L. cv. Pink Pearl Cultured in vitro
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Lee Kyung-Soon
Churg Chung-Han
Chung Yong-Mo
Kim Do-Hoon
Jung Soon-Jae
Nam Jae-Sung
Kim Gyung-Tae
Yi Young-Byung
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Abstract
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The regeneration and growth of bulblets from the bulb scale segments of Hyacinthus orientalis L. cv. Pink Pearl were more efficient in IBA than IAA at the same concentrations (1.0 and 3.0 mg/l).. The normal (base-down) orientation of explants was more effective for bulblet regeneration and root growth than the inverted (base-up) orientation. The growth of bulblets and roots was increased higher in the perlite than the agar medium. These results suggested that the alternate culture system, first cultured in the agar medium for bulblet regeneration, and then in the perlite medium for bulblet growth, may be more useful for efficient in vitro culture of hyacinth (H. orientalils) cv. Pink Pearl.
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KEYWORD
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Bulblet regeneration and growth, explant orientation, Hyacinthus orientalis, IBA, perlite
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